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MICROSCOPY

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With
increased resolution of digital cameras today the ability to
capture quality images through a microscope is now possible and an
inexpensive solution to microscopy.
University
researchers, students, and hobbyist will find connecting
your digital camera to various microscopes is both productive and
rewarding. You will find the LE-Adapter
is a tremendous asset!
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Sony W1
Microscope |
LensPlus has recently completed a review of the
new Sony W1 Digital Camera and how is compares to our
LE-Adapters. We have found it works extremely well with our
products.
Click HERE for
an in-depth look at this great combination. |
CSU
Stanislaus Botany Image Collection
Steve Wolf - CSU Stanislaus
(Steve's website contains images taken
with the LE-Adapter)
I use a Nikon 990 digital
camera and trinocular compound and dissecting scopes with wide field
eyepieces. The camera is attached to the scope via a LE-Adapter
available at
http://www.lensadapter.net
. If you don't have a trinocular you can
just mount the camera on one of the eyepieces. Below is a photo of the
set up I use. I find it much easier to work via a TV monitor. I use the
video out from the camera into a TV with an RCA jack. This way you see
everything that appears on the camera LCD. I also recommend using the AC
adapter, otherwise you run through batteries very quickly and the LCD
times out. You must use the Nikon remote to eliminate camera
shake. You can't use the self timer because that only works in macro
mode. I shoot in the infinity mode and focus via the microscope. You do
not want the camera to try to focus because it rarely gets it right. I
mostly use the program mode (A-REC). Vignetting is a common problem.
You can reduce it in two ways: 1. use the
optical zoom 2. use a relatively large aperture (small f-stop). Take
several photos of each slide and slightly vary the microscope focus each
time. This is one of the main advantages of a digital camera, not having
to pay for film and developing! For "web publishing" I take the photos
at 1024 x 768 and crop them to 800 x 600. I save in jpeg format with
medium compression. Microscope lamps vary considerably and the color
temperature changes depending on how high you turn up the lamp. Play
with the white balance and light levels. Once you find the most accurate
stick with those settings.
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